Instrument Hub

 

 

Agilent 6410 Triple Quadrupole

LCMS

The Mass Spectrometers in the Auckland Cancer Society Research Centre analyze targeted small molecule in complex samples including drug extraction from blood and tissues, and analysing drugs and their breakdown products.

Related Instruments

 

 

Overview

In the development of anti-cancer drugs, it is increasingly important to measure their concentrations accurately in blood and tissues. The ACSRC has extensive facilities for extracting drugs from blood and tissues, and analysing the concentrations of the drug and its breakdown products (which are usually metabolites produced in the body). We offer world class training in the use of high performance liquid chromatography and mass spectrometry. Our bioanalysis cluster provides support for in vitro and in vivo pharmacokinetic studies in the development of our drugs, as well as servicing other departments in the University.

These systems combine the separation power of HPLC with the detection power (i.e. sensitivity and selectivity) of a triple quad instrument for targeted analysis of small molecules in complex samples. The Multimode Ionization (MMI) source incorporates both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) into a single ion source. This makes it well equipped for samples with a wide diversity of analytes and where high throughput is needed. The simplicity of the instruments make them ideally suited for walk-up, shared-access.

 

    Features

      • Mass Range: 5-2,000 m/z
      • Mass Resolution: 0.7 amu (FWHM)
      • Ionization Sources: ESI & APCI
      • In-line UV/Vis absorbance detector

Software

Analysis for the Agilent Mass Spectrometers is supported by the MassHunter software.

Contact Sree Sreebhavan for more information.

Support

Email Sree Sreebhavan for all enquiries related to the Agilent 6140 Mass Spectrometer.

Considerations

Suitable for reverse-phase HPLC using columns with i.d. of 2 – 4.6 mm.The instrument is located in a standard (PC1) lab and standard lab requirements are in place.

Limitations

This is a low-resolution mass spec instrument with an m/z range up to 2,000.

Cost

These instruments are charged per hour. Current rates are:

Equipment Non-commercial (rate/hour) Commercial (rate/hour)
QQQ $60.00 $90.00
LC/MS $40.00 $60.00
HPLC $18.00 $27.00

Eligibility

Available for postgraduate students, doctoral candidates and staff of the University of Auckland. External users and commercial users are also eligible to use the equipment.

Access Requirements

The Agilent Mass Spectrometers are housed on the Auckland Cancer Society Research Centre in the Faculty of Health and Medical Sciences.

Training

This instrument is available for self-use. Users are required to undergo training (provided) before using the equipment, and should contact Sree Sreebhavan to arrange details.

Booking

Contact Sree Sreebhavan for all instrument bookings.