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Liggins Triple Quadruople LC-MS

Triple quadrupole mass spectrometry is used to measure the concentration of targeted small molecules in complex mixtures. Available analyses include steroids, vitamins, amino acids and similar molecules.

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Overview

Two different triple quadrupole mass spectrometers are available at the Liggins Institute, a TSQ Quantiva (Thermo) and an older TSQ Quantum Ultra AM (Thermo). Each has an Ultra High Pressure Chromatography (UPLC) system for sample delivery and separation. 

Features

– Single Reaction Monitoring (SRM) mode to ‘target’ and quantify specific compounds in sample extracts.
– High mass accuracy and specificity to differentiate between very similar molecules.
– Automated sample delivery from vials or 96 well plates to enable overnight analyses to be undertaken.
– Can be used for a variety of matrices (following sample extraction) eg. plasma, saliva, urine, hair, milk and blood.

Software

Analysis for the Liggins Triple Quadrupole data is supported by Xcaliber and other software available on the Q-Exactive Virtual Machine.

Support

Email Eric Thorstensen or Kalita Prangnell for all enquiries related to Triple Quadrupole MS.

Considerations

There are several small molecule analysis ‘panels’ which have been fully validated and published in scientific journals; these are available to users as “off the shelf” products. De novo assay setup is also available and should be discussed with the Liggins Institute mass spectrometry technical team.

Limitations

Not suitable for proteins and large compounds, unless they have been enzymatically digested.

Cost

The cost of each analysis will vary from project to project depending on the analysis required and the number of samples. Contact Eric Thorstensen to discuss your project and for costings estimates.

Eligibility

Post-graduate Students, Doctoral Candidates, Research/Academic Staff, Professional Staff, Collaborators (external to the University), Commercial clients.

Access Requirements

The Triple Quadrupole LC-MS instruments are housed on the Grafton campus in the Liggins Institute.

Training

These instruments are operated by Kalita Prangnell.

Booking

Arranged through Eric Thorstensen.