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Overview

The ZenoTOF 7600 delivers a powerful combination of high sensitivity, speed, and resolution enabling analyses of challenging and complex samples. Ultra-fast acquisition rates allow you to collect high-resolution MS/MS data on every detectable analyte in your sample, giving you the data you need to advance your research. It is ideally suited for protein identification, PTM analysis, quantitative global proteomics, protein MW analysis.

The Zeno trapping function boosts MS/MS sensitivity at least 5-fold across the mass range – and up to 20-fold for lower mass fragment ions <m/z 300.

In addition to the usual CID mode of fragmentation, the ZenoTOF also offers the highly versatile Electron Activated Dissociation (EAD) mode, which utilises a tuneable kinetic energy electron beam to deliver an alternative fragmentation mode for a variety of analytes (lipids, peptides, intact proteins, labile PTMs, even singly-charged analytes).

Given the high sensitivity on offer, short data acquisition times can be used for Variable Window SWATH Acquisition, allowing thousands of proteins to be quantified in each sample, even when using very short LC run times. With a dynamic range in excess of five orders of magnitude, and up to 133 MS/MS events per second, this is our most powerful instrument for proteomic applications.

    Features

• • • Mass Range: 5-40,000 m/z
    • Mass Selection Range: 5-2250 m/z
    • Mass Accuracy : 2 ppm
    • Mass Resolution: ≥42,000 (FWHM) at 956 m/z
    • Capillary LC system
    •  

Software

Analysis for the ZenoTOF 7600 is supported by the following software:

• ProteinPilot
• PeakView
• MultiQuant
• MarkerView
• Skyline
• DIA-NN

Access to the above software is available in the analysis suit or via the  Proteomics Virtual Machine. Contact Martin Middleditch for more information.

Support

Email Martin Middleditch for all enquiries related to the ZenoTOF 7600 MS.

Considerations

• Sample preparation protocols for each project should preferably be discussed beforehand for quality data analysis.
• Sample preparation (e.g. cell culture) involving serum albumin should be avoided where possible as the albumin can suppress other scarce proteins/peptides.
• While LC-MS does deal with some level of salts, it is best to minimise concentrations of non-volatile buffers.
• It is best if no detergents are used during sample prep, or extensive steps taken to massively reduce their concentration in the final extract.
• This instrument is not housed in a PC1 area and only non-hazardous samples are allowed to be transported to and analysed in the laboratory.

Limitations

• This instrument is run primarily by centre staff, but can be made available for self-use for researchers presenting with a large block of work.
• Protein identification and quantification can be compromised if the sample preparation and processing are inadequate.
• Data analysis can be complex and time-consuming depending on the application.
• The instrument is set up for Capillary flow chromatography; CID and EAD fragmentation processes are available.

Cost

The cost varies depending on task and support level required. Current user costs are listed in iLab booking system.

Eligibility

Available for postgraduate students, doctoral candidates and staff of the University of Auckland. External users and commercial users are also eligible to use the equipment.

Access Requirements

The ZenoTOF 7600 is housed in Building 301 in the City Campus Science Centre

Training

Training to access lower-cost self-use services, are only available for users with large blocks of work.

Booking

Users can request analysis on the ZenoTOF through ou iLab booking system. Users and their principal investigators or research group need to first have accounts in the iLab booking system. Contact Martin for more details.