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Overview

The TripleTOF 6600 is a versatile instrument delivering a combination of high sensitivity, speed, and productivity in a single platform to help you dig deeper into complex samples. Fast acquisition rates allow you to collect high-resolution MS/MS data on every detectable analyte in your sample, giving you the data you need to advance your research. It is ideally suited for protein identification, PTM analysis, quantitative global proteomics, protein MW analysis. Offline fractionation (e.g. Strong Cation Exchange) coupled with reversed phase LC-MS/MS analysis enables the in-depth analysis of complex samples, while advanced techniques (SWATH, scheduled MRM, PRM) allow you to get the most information out of your sample.

This instrument offers state-of-the-art features including Variable Window SWATH Acquisition for comprehensive proteomic analysis, and high-resolution Multiple Reaction Monitoring (MRM-HR) for accurate quantitation of analytes in highly complex samples. With a dynamic range in excess of five orders of magnitude, and up to 133 MS/MS events per second, this is a powerful instrument for proteomic applications.

    Features

• • • Mass Range: 5-40,000 m/z
    • Mass Selection Range: 5-2250 m/z
    • Mass Accuracy : 2 ppm
    • Mass Resolution: ≥35,000 (FWHM) at 956 m/z
    • Capillary LC and NanoESI
    • 4-channel MCP detector with ADC signal processing for extended dynamic range

Software

Analysis for the TripleTOF 6600 is supported by the following software:

• ProteinPilot
• PeakView
• MultiQuant
• MarkerView
• Skyline
• DIA-NN

Access to the above software is available in the analysis suit or via the  Proteomics Virtual Machine. Contact Martin Middleditch for more information.

Support

Email Martin Middleditch for all enquiries related to the TripleTOF 6600 MS.

Considerations

• Sample preparation protocols for each project should preferably be discussed beforehand for quality data analysis.
• Sample preparation involving serum albumin should be avoided where possible as the albumin can suppress other scarce proteins/peptides.
• While LC-MS does deal with some level of salts, it is best to minimise concentrations of non-volatile buffers.
• It is best if no detergents are used during sample prep, or extensive steps taken to massively reduce their concentration in the final extract.
• This instrument is not housed in a PC1 area and only non-hazardous samples are allowed to be transported to and analyzed in the laboratory.

Limitations

• This instrument is run primarily by centre staff, but can be made available for self-use for researchers presenting with a large block of work.
• Protein identification and quantification can be compromised if the sample preparation and processing are inadequate.
• Data analysis can be complex and time-consuming depending on the application.
• The instrument is set up for Capillary flow chromatography; CID is the only fragmentation process available.

Cost

The cost varies depending on task and support level required. Current user costs are listed in iLab booking system.

Eligibility

Available for postgraduate students, doctoral candidates and staff of the University of Auckland. External users and commercial users are also eligible to use the equipment.

Access Requirements

The TripleTOF6600 is housed in Building 301 in the City Campus Science Centre.

Training

Training to access lower-cost self-use services, are only available for users with large blocks of work.

Booking

Users can request analysis on the TripleTOF through ou iLab booking system. Users and their principal investigators or research group need to first have accounts in the iLab booking system. Contact Martin for more details.