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Agilent 6530B Q-TOF LC-MS/MS (ACSRC)
The Agilent 6530B Q-TOF LC-MS/MS is a high resolution mass spectrometer instrument suitable for analysis of multiple small molecule targets in complex samples as well as characterisation of intact protein, including antibodies.
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Overview
The Agilent 6530B Q-TOF LC-MS/MS combines the separation power of High Performance Liquid Chromatography (HPLC) with the higher mass accuracy and detection power of a Q-TOF instrument and provides high levels of sensitivity and selectivity for targeted analysis of small molecules in complex samples such as tissue homogenates, blood, or lysates from cell cultures. The electrospray ionization (ESI) source is equipped with Agilent Jet Stream technology that uses super-heated nitrogen to improve ion generation and desolvation, which leads to greater signal and reduced noise. The higher mass accuracy enables analysis of multiple small molecule targets in complex samples as well as characterisation of intact protein, including antibodies.
Features
- Mass Range: 50-20,000 m/z
- Mass Resolution: >20,000 MS; >10,000 MS/MS
- Ionization Sources: ESI
- In-line UV/Vis absorbance detector
Software
Analysis for the Agilent Mass Spectrometers is supported by MassHunter and BioConfrm v10 software.
Contact Sree Sreebhavan for more information.
Access to the software is available on the Small molecules Virtual Machine.
Support
Email Sree Sreebhavan for all enquiries related to the Agilent 6530 Q-TOF Mass Spectrometer.
Considerations
Suitable for reverse-phase HPLC using columns of 2 – 4.6 mm i.d. The instrument is located in a standard (PC1) lab and standard lab requirements are in place.
Limitations
This is a high-resolution mass spec instrument with an m/z range up to 20,000.
Cost
This instrument is charged per hour. Current rates are:
- $60/h (Public Good and Non-Commercial)
- $90/h (Commercial)
Contact Sree Sreebhavan for more information.
Eligibility
Available for postgraduate students, doctoral candidates and staff of the University of Auckland. External users and commercial users are also eligible to use the equipment.
Access Requirements
The 6530B Q-TOF LC-MS/MS is housed in Room 503-318 in the Faculty of Medical and Health Sciences and operated & managed through the Auckland Cancer Society Research Centre.
Training
This instrument is available for self-use only and not operated as a service. Users are required to undergo basic training (provided) before using the equipment, and should contact Sree Sreebhavan to arrange details.
Booking
Contact Sree Sreebhavan for all instrument bookings.