Instrument Hub
Agilent 6460A Triple Quadrupole
LCMS (ACSRC)
The Agilent 6460A Triple Quadrupole LCMS is a low resolution mass spectrometer instrument suitable for targeted analysis of small molecules in complex samples.
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Overview
The Agilent 6460A Triple Quadrupole LCMS combines the separation power of High Performance Liquid Chromatography (HPLC) with the detection power of a triple quad instrument and provides the highest levels of sensitivity and selectivity for targeted analysis of small molecules in complex samples such as tissue homogenates, blood, or lysates from cell cultures. The electrospray ionization (ESI) source is equipped with Agilent Jet Stream technology that uses super-heated nitrogen to improve ion generation and desolvation, which leads to greater signal and reduced noise. This instrument is well suited for small polar molecules and metabolites as well as targeted proteomics applications of samples digested with proteases such as trypsin.
Features
- Mass Range: 5-3,000 m/z
- Mass Resolution: 0.7 amu (FWHM)
- Ionization Sources: ESI
- In-line UV/Vis absorbance detector
Software
Analysis for the Agilent Mass Spectrometers is supported by MassHunter software.
Contact Sree Sreebhavan for more information.
Access to the software is available on the Small molecules Virtual Machine.
Support
Email Sree Sreebhavan for all enquiries related to the Agilent 6460 Mass Spectrometer.
Considerations
Suitable for reverse-phase HPLC using columns of 2 – 4.6 mm i.d. The instrument is located in a standard (PC1) lab and standard lab requirements are in place.
Limitations
This is a low-resolution mass spec instrument with an m/z range up to 3,000.
Cost
This instrument is charged per hour. Current rates are:
- $60/h (Public Good and Non-Commercial)
- $90/h (Commercial)
Contact Sree Sreebhavan for more information.
Eligibility
Available for postgraduate students, doctoral candidates and staff of the University of Auckland. External users and commercial users are also eligible to use the equipment.
Access Requirements
The Agilent 6460A Triple Quadrupole LCMS is housed in Room 504-011 in the Auckland Cancer Society Research Centre in the Faculty of Medical and Health Sciences.
Training
This instrument is available for self-use only and not operated as a service. Users are required to undergo basic training (provided) before using the equipment, and should contact Sree Sreebhavan to arrange details.
Booking
Contact Sree Sreebhavan for all instrument bookings.