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Agilent 6135BA MSD XT Single Quadrupole

LC-MS (ACSRC)

The Agilent 6135BA MSD XT Single Quadrupole LC-MS is a low-resolution mass spectrometer instrument suitable for targeted analysis of small molecules in relatively clean samples.

Overview

The Agilent 6135BA MSD XT Single Quadrupole LC-MS combines the separation power of High Performance Liquid Chromatography (HPLC) with the detection power (i.e. sensitivity and selectivity) of a single quad instrument for targeted analysis of small molecules in less complex samples or when high background is not an issue because of relative high concentrations of the target analyte(s). The instrument can be configured with either an electrospray ionization (ESI) or an atmospheric pressure chemical ionization (APCI) ion source.

Features

  • Mass Range: 2-3,000 m/z
  • Mass Resolution: <0.7 amu (FWHM)
  • Ionization Sources: ESI & APCI
  • In-line UV/Vis absorbance detector

Software

Analysis for the Agilent Mass Spectrometers is supported by OpenLab CDS ChemStation Edition software.

Contact Sree Sreebhavan for more information.

Access to the software is available on the Small molecules Virtual Machine.

Support

Email Sree Sreebhavan for all enquiries related to the Agilent 6135 Mass Spectrometer.

Considerations

Suitable for reverse-phase HPLC using columns of 2 – 4.6 mm i.d. The instrument is located in a standard (PC1) lab and standard lab requirements are in place.

Limitations

This is a low-resolution mass spec instrument with an m/z range up to 3,000. Single-stage MS produces lower signal-to-noise ratios, which can be a limitation in samples with high background and/or low target concentration.

Cost

This instrument is charged per hour. Current rates are:

  • $40/h (Public Good and Non-Commercial)
  • $60/h (Commercial)

Contact Sree Sreebhavan for more information.

Eligibility

Available for postgraduate students, doctoral candidates and staff of the University of Auckland. External users and commercial users are also eligible to use the equipment.

Access Requirements

The Agilent 6135BA MSD XT Single Quadrupole LC-MS is housed in Room 503-318 in the Faculty of Medical and Health Sciences and operated & managed through the Auckland Cancer Society Research Centre.

Training

This instrument is available for self-use only and not operated as a service. Users are required to undergo basic training (provided) before using the equipment, and should contact Sree Sreebhavan to arrange details.

Booking

Contact Sree Sreebhavan for all instrument bookings.